Molecular Cloning has served as the
foundation of technical expertise in labs worldwide for 30 years.
No other manual has been so popular, or so influential. Molecular
Cloning, Fourth Edition, by the celebrated founding author Joe
Sambrook and new co-author, the distinguished HHMI investigator
Michael Green, preserves the highly praised detail and clarity of
previous editions and includes specific chapters and protocols
commissioned for the book from expert practitioners at Yale, U
Mass, Rockefeller University, Texas Tech, Cold Spring Harbor
Laboratory, Washington University, and other leading institutions.
The theoretical and historical underpinnings of techniques are
prominent features of the presentation throughout, information that
does much to help trouble-shoot experimental problems.
For the fourth edition of this classic work,
the content has been entirely recast to include nucleic-acid based
methods selected as the most widely used and valuable in molecular
and cellular biology laboratories.
Core chapters from the third edition have
been revised to feature current strategies and approaches to the
preparation and cloning of nucleic acids, gene transfer, and
expression analysis. They are augmented by 12 new chapters which
show how DNA, RNA, and proteins should be prepared, evaluated, and
manipulated, and how data generation and analysis can be
handled.
The new content includes methods for studying
interactions between cellular components, such as microarrays,
next-generation sequencing technologies, RNA interference, and
epigenetic analysis using DNA methylation techniques and chromatin
immunoprecipitation. To make sense of the wealth of data produced
by these techniques, a bioinformatics chapter describes the use of
analytical tools for comparing sequences of genes and proteins and
identifying common expression patterns among sets of genes.
Building on thirty years of trust, reliability,
and authority, the fourth edition of Molecular Cloning is the new
gold standard—the one indispensable molecular biology laboratory
manual and reference source.
新版特点:
? Extensive new content: 12 entirely new chapters are devoted to
the most exciting current research strategies, including epigenetic
analysis, RNA interference, genome sequencing, and
bioinformatics
? Expanded scope: the nucleic-acid-based techniques selected for
inclusion have promoted recent advances in gene transfer, protein
expression, RNA analysis, and expression of cloned genes
? Classic content: 10 original core chapters have been updated
to reflect developments and innovations in standard techniques and
to introduce new cutting-edge protocols
? Easy-to-follow format: the previous editions’ renowned attention
to detail and accuracy are fully retained
? Essential appendices: an up-to-date collection of reagents,
vectors, media, detection systems, and commonly used techniques are
included
? Expanded authorship: chapters and protocols have been
specifically commissioned from renowned experts at leading
institutions
關於作者：
JOSEPH SAMBROOK：
Sambrook was educated at the University of Liverpool BSc hons
1962 and obtained his PhD at the Australian National University in
1966. He did postdoctoral research at the MRC Laboratory of
Molecular Biology 1966-67 and the Salk Institute for Biological
Studies 1967-69. In 1969 he was hired by James D. Watson to work
at the Cold Spring Harbor Laboratory in New York. Watson has been
reported to say this was the best hiring decision he ever made. Joe
was responsible for creating a combative creative environment at
CSHL that fomented discovery. Subsequently he worked at the
University of Texas Southwestern Medical Center Dallas.
Sambrook is best known for his studies on DNA tumor viruses and
the molecular biology of normal and neoplastic cells. His Tumour
Virus Group at Cold Spring Harbor identified and mapped all of the
major genes of adenoviruses and SV40, determined their
transcriptional control in infected and transformed cells, and
elucidated the mechanism of integration of these viruses into the
genome of the host cell.[citation needed] He has also made
important contributions to the understanding of intracellular
traffic and protein folding and is an influential leader in the
field of the molecular genetics of human cancer.
目錄：
VOLUME 1
Part 1: Essentials
1. Isolation and Quantification of DNA
2. Analysis of DNA
3. Cloning and Transformation with Plasmid Vectors
4. Gateway Recombinational Cloning
John S. Reece-Hoyes and Albertha J.M. Walhout
5. Working with Bacterial Artificial Chromosomes and Other
High-Capacity Vectors
Nathaniel Heintz and Shiaoching Gong
6. Extraction, Purification, and Analysis of RNA from Eukaryotic
Cells
7. Polymerase Chain Reaction
8. Bioinformatics
Jui-Hung Hung and Zhiping Weng
VOLUME 2
Part 2: Analysis and Manipulation of DNA and RNA
9. Quantification of DNA and RNA by Real-Time Polymerase Chain
Reaction
10. Nucleic Acid Platform Technologies
Oliver Rando
11. DNA Sequencing
Elaine Mardis and W. Richard McCombie
12. Analysis of DNA Methylation in Mammalian Cells
Paul M. Lizardi, Qin Yan, and Narendra Wajapeyee
13. Preparation of Labeled DNA, RNA, and Oligonucleotide
Probes
14. Methods for In Vitro Mutagenesis
Matteo Forloni, Alex Liu, and Narendra Wajapeyee
Part 3: Introducing Genes into Cells
15. Introducing Genes into Cultured Mammalian Cells
Priti Kumar, Arvindhan Nagarajan, and Pradeep D. Uchil
16. Introducing Genes into Mammalian Cells: Viral Vectors
Guangping Gao and Miguel Sena-Esteves
VOLUME 3
Part 4: Gene Expression
17. Analysis of Gene Regulation Using Reporter Systems
Pradeep D. Uchil, Arvindhan Nagarajan, and Priti Kumar
18. RNA Interference and Small RNA Analysis
Chengjian Li and Phillip D. Zamore
19. Expressing Cloned Genes for Protein Production, Purification,
and Analysis
Clara L. Kielkopf, William Bauer, and Ina Urbatsch
Part 5: Interaction Analysis
20. Cross-Linking Technologies for Analysis of Chromatin Structure
and Function
Tae Hoon Kim and Job Dekker
21. Mapping of In Vivo RNA-Binding Sites by UV-Cross-Linking
Immunoprecipitation CLIP
Jennifer C. Darnell, Aldo Mele, Ka Ying Sharon Hung, and Robert B.
Darnell
22. Gateway-Compatible Yeast One-Hybrid and Two-Hybrid Assays
John S. Reece-Hoyes and Albertha J.M. Walhout
Appendices
1. Reagents and Buffers
2. Commonly Used Techniques
3. Detection Systems
4. General Safety and Hazardous Material
內容試閱：
General Safety and Hazardous Material InformationbrThis
manual should be used by laboratory personnel with experience in
laboratory and chemicalbrsafety or students under the
supervision of suitably trained personnel. The procedures,
chemicals,brand equipment referenced in this manual are
hazardous and can cause serious injury unless per-brformed,
handled, and used with care and in a manner consistent with safe
laboratory practices.brStudents and researchers using the
procedures in this manual do so at their own risk. It is
essentialbrfor your safety that you consult the appropriate
Material Safety Data Sheets, the manufacturers’brmanuals
accompanying equipment, and your institution’s Environmental Health
and SafetybrOffice, as well as the General Safety and
Disposal Information in Appendix 4 for proper handlingbrof
hazardous materials described in this manual. Cold Spring Harbor
Laboratory makes no repre-brsentations or warranties with
respect to the material set forth in this manual and has no
liability inbrconnection with the use of these
materials.brAll registered trademarks, trade names, and
brand names mentioned in this book are the prop-brerty of
the respective owners. Readers should consult individual
manufacturers and other resour-brces for current and
specific product information. Appropriate sources for obtaining
safetybrinformation and general guidelines for laboratory
safety are provided in the General Safety andbrHazardous
Material appendix of this manual.
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