目录
章 生物化学常规技术训练 1
实验1 蛋白质的沉淀和分离——离心技术 1
Chapter I Routine Technical Training in Biochemistry 4
Experiment 1 Precipitation and Separation of Proteins—Operation of Centrifugation 4
实验2 硫酸铜的浓度测定——分光光度法 7
Experiment 2 Determination of CuSO4 Concentration—Spectrophotometry Technique 12
实验3 血清蛋白乙酸纤维素膜电泳定量分析 17
Experiment 3 Quantitative Analysis of Serum Proteins Using Cellulose Acetate Membrane Electrophoresis 27
实验4 离子交换层析 38
Experiment 4 Ion Exchange Chromatography 41
第二章 基础性实验 45
实验5 蛋白质的盐析与透析 45
Chapter II Fundamental Experiments 47
Experiment 5 Salt Out and Dialysis of Proteins 47
实验6 双缩脲法测定血清蛋白总量 49
Experiment 6 Determining the Total Protein in Serum Using the Biuret Method 51
实验7 酪蛋白等电点测定 53
Experiment 7 Determining the Isoelectric Point of Casein 55
实验8 唾液淀粉酶专一性以及多种因素对酶活性的影响 57
Experiment 8 Specificity of Salivary Amylase and the Factors Affecting Enzyme Activities 60
实验9 乳酸脱氢酶的递氢作用 63
Experiment 9 The Hydrogen Transferring Effect of Lactate Dehydrogenase 65
实验10 血清胆固醇总量的测定（硫铁磷法） 67
Experiment 10 Determining Total Serum Cholesterol (By Sulphur-Iron-Phosphorus Reagent) 69
实验11 血清谷丙转氨酶（GPT）活性测定 71
Experiment 11 Determining Glutamic-Pyruvic Transaminase (GPT) Activity in Serum 73
实验12 血清二氧化碳结合力的测定——Van Slyke酸碱滴定法 75
Experiment 12 Determining CO2 Combining Power in Serum—Van Slyke Acid-Base Titration 77
实验13 肝脏中酮体的生成和检测 79
Experiment 13 Production and Detection of Ketone Bodies in the Liver 81
实验14 PCR扩增髓鞘碱性蛋白基因 83
Experiment 14 Amplification of the Myelin Basic Protein Gene Using PCR 85
实验15 DNA琼脂糖凝胶电泳 87
Experiment 15 DNA Agarose Gel Electrophoresis 89
第三章 综合性实验 91
实验16 血清免疫球蛋白的分离、纯化和鉴定 91
Chapter III Integrated Experiments 94
Experiment 16 Separation， Purification and Identification of Serum Immunoglobulin 94
实验17 碱性磷酸酶的分离纯化及其Km值测定 97
Experiment 17 Isolating Alkaline Phosphatase and Determining It’s Km 101
实验18 质粒DNA的分离纯化与鉴定 106
Experiment 18 Isolation， Purification and Identification of Plasmid DNA 108
实验19 动物肝脏中DNA的提取和检测 110
Experiment 19 DNA Extraction and Detection from AnimalLiver 112
实验20 血清IgG的SDS-PAGE鉴定 114
Experiment 20 Identifying Serum IgGUsing SDS-PAGE 118
实验21 人肝癌细胞株HepG2中内参GAPDH的免疫印迹检 122
Experiment 21 Detecting GAPDH in the Human Hepatoma Cell Line HepG2 Using a Western Blotting 126
实验22 运动对骨骼肌糖代谢的影响 130
Experiment 22 Effects of Exercise on Glycometabolism in Skeletal Muscle 133
实验23 水果中维生素C的含量测定及温度、碱和重金属对样品中维生素C影响的动态观察 136
Experiment 23 Determination of Vitamin C in Fruits and Dynamic Observation of the Effects of Temperature， Alkali and Heavy Metals on Vitamin C in Samples 140
第四章 设计性实验 144
实验24 基因克隆技术：人髓鞘碱性蛋白基因重组质粒的构建与鉴定 144
Chapter IV Design Experiments 148
Experiment 24 Gene Cloning—Construction and Identification of the Recombinant Plasmid of the Human Myelin Basic Protein Gene 148
实验25 人基因多态性分析 151
Experiment 25 Gene Polymorphism Analysis of Humans 156
附录 实验室安全须知 161
Appendix Laboratory Safety Procedures 161
参考文献 162
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